Search results for "vesicular genomic DNA"

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Kinetics and Topology of DNA Associated with Circulating Extracellular Vesicles Released during Exercise.

2021

AbstractAlthough it is widely accepted that cancer derived extracellular vesicles (EVs) carry DNA cargo, the association of cell-free circulating DNA (cfDNA) and EVs in plasma of healthy humans remains elusive. Using a physiological exercise model, where EVs and cfDNA are synchronously released, we aimed to characterize the kinetics and localization of DNA associated with EVs. EVs were separated from human plasma using size exclusion chromatography or immuno-affinity capture for CD9+, CD63+, and CD81+ EVs. DNA was quantified with an ultra-sensitive qPCR assay targeting repetitive LINE elements, with or without DNase digestion. This model shows that a minute part of circulating cell-free DNA…

AdultMalelcsh:QH426-470KineticsexosomesExtracellular vesiclesPolymerase Chain ReactionArticle796 Athletic and outdoor sports and games570 Life sciencescell-free DNAchemistry.chemical_compoundExtracellular VesiclesYoung Adultphysical exerciseHumansExerciseCD63human plasma796 SportChemistryHealthy VolunteersCell biologylcsh:GeneticsKineticsLong Interspersed Nucleotide ElementsHuman plasmaChromatography GelCirculating DNAFemalecoronavesicular genomic DNACell-Free Nucleic AcidsDNACD81extracellular DNAintraluminal570 BiowissenschaftenGenes

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PEPhysical Sciences and Engineering
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